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Atonal instruments cannot be used to play the passtune. When you apply an atonal instrument, you do not get a "Improvise? (yn)" prompt.

Atonal instruments in NetHack 3.4.3 are:

The main use of these is waking up monsters. The non magical variants of these have little other use, but do have some use, for example, enabling an Elbereth square.

In NetHack, whistles are not instruments.

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